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> home > Products > Primary Antibodies > Antibody Collections > Testis positive > JLP/MAX binding protein Polyclonal Antibody
JLP/MAX binding protein Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| IHC-P, IHC-F, IF, ICC, E |
|---|---|
| Primary Accession | O60271 |
| Reactivity | Rat, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 146 KDa |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human JLP/JIP-4 |
| Epitope Specificity | 101-188/1321 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. |
| SIMILARITY | Belongs to the JIP scaffold family. |
| SUBUNIT | Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1. |
| Post-translational modifications | Phosphorylated by MAPK8 and MAPK14 (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | JIP-4 is a 1,321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state. |
| Gene ID | 9043 |
|---|---|
| Other Names | C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, CT89, Human lung cancer oncogene 6 protein, HLC-6, JNK-associated leucine-zipper protein, JLP, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, PHET, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, SPAG9 (HGNC:14524) |
| Target/Specificity | Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients. |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Format | 0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
| Name | SPAG9 (HGNC:14524) |
|---|---|
| Function | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein- dynactin complex (PubMed:29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q58A65}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q58A65}. Lysosome membrane. Note=Perinuclear distribution in response to stress signals such as UV radiation {ECO:0000250|UniProtKB:Q58A65} |
| Tissue Location | Expressed only in testis on the round spermatids of stage I, II and II. Absent in spermatogonia and spermatocyte [Isoform 3]: Expressed in testis. |
Research Areas
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$ 385.00
Cat# AP54804
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